Reaction Velocity of an Enzymatic Reaction
Reaction velocity versus substrate concentration in an enzymatic reaction involving untreated cells or cells treated with Puromycin.
Whether the cell was treated.
Substrate concentrations (ppm).
Instantaneous reaction rates (counts/min/min).
Data on the velocity of an enzymatic reaction were obtained by Treloar (1974). The number of counts per minute of radioactive product from the reaction was measured as a function of substrate concentration in parts per million (ppm) and from these counts the initial rate (or velocity) of the reaction was calculated (counts/min/min). The experiment was conducted once with the enzyme treated with Puromycin, and once with the enzyme untreated.
The data are given in Box & Jenkins (1976). Obtained from the Time Series Data Library at http://www-personal.buseco.monash.edu.au/~hyndman/TSDL/.
Bates, D.M. and Watts, D.G. (1988). Nonlinear Regression Analysis and Its Applications. New York: Wiley. Appendix A1.3.
Treloar, M. A. (1974). Effects of Puromycin on Galactosyltransferase in Golgi Membranes. M.Sc. Thesis, U. of Toronto.
t = octave.dataset.Puromycin; # TODO: Port example to Octave